Performance Characteristics of a Quantitative, Homogeneous TaqMan RT-PCR Test for HCV RNA
نویسندگان
چکیده
منابع مشابه
A universal TaqMan-based RT-PCR protocol for cost-efficient detection of small noncoding RNA.
Several methods for the detection of RNA have been developed over time. For small RNA detection, a stem-loop reverse primer-based protocol relying on TaqMan RT-PCR has been described. This protocol requires an individual specific TaqMan probe for each target RNA and, hence, is highly cost-intensive for experiments with small sample sizes or large numbers of different samples. We describe a univ...
متن کاملGuidelines for quantitative rt-PCR.
Something I notice often in supervising new graduate students is their frustration with the irreproducibility of their biological data. For example, I am asked frequently ‘‘how many biological replicates do I need for qRT-PCR’’? The answer, of course, lies first with the students themselves. They need as many replicates as will persuade them of the validity of their observations. However, for m...
متن کاملHeparinase treatment of RNA before quantitative real-time RT-PCR.
Vol. 35, No. 6 (2003) BioTechniques 1141 the ABI PRISM® 7000 and the TaqMan Universal PCR Master Mix protocols (Applied Biosystems). After discovering that the RNA from the thecal cells had vascular endothelial growth factor (VEGF) amplification and that RNA from granulosa cells had no VEGF amplification, the 18S rRNA amplification (TaqMan Pre-Developed Assay Reagents; Applied Biosystems), whic...
متن کاملThe impact of storage time on HCV RNA quantitative detection in serum samples by TaqMan real-time PCR
Information on the concentration of hepatitis C virus (HCV) RNA is useful for patient management, including assessing treatment initiation and treatment response, and monitoring follow-up. Previous studies have reported that poor sample processing and storage conditions might influence the stability of HCV RNA, and hence its detectability. We analyzed 30 patients known to be positive with high/...
متن کاملA quantitative PCR (TaqMan) assay for pathogenic Leptospira spp
BACKGROUND Leptospirosis is an emerging infectious disease. The differential diagnosis of leptospirosis is difficult due to the varied and often "flu like" symptoms which may result in a missed or delayed diagnosis. There are over 230 known serovars in the genus Leptospira. Confirmatory serological diagnosis of leptospirosis is usually made using the microscopic agglutination test (MAT) which r...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: The Journal of Molecular Diagnostics
سال: 2000
ISSN: 1525-1578
DOI: 10.1016/s1525-1578(10)60632-0